Porcine Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial, CLPP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class 3.4.21.92,Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial),Endopeptidase Clp,CLPP,ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit; PRLTS3; ATP-dependent protease ClpAP, proteolytic subunit, Porcine; endopeptidase Clp; caseinolytic mitochondrial matrix peptidase proteolytic subunit
Search name
Porcine CLPP ELISA KIT ,Porcine ClpP caseinolytic protease ELISA KIT ,Porcine ATP-dependent ELISA KIT ,Porcine proteolytic subunit homolog ELISA KIT ,Porcine ClpP caseinolytic peptidase ELISA KIT ,Porcine ATP-dependent ELISA KIT ,Porcine proteolytic subunit homolog ELISA KIT ,Porcine ClpP caseinolytic peptidase ATP-dependent ELISA KIT ,Porcine proteolytic subunit ELISA KIT ,Porcine PRLTS3 ELISA KIT ,Porcine ATP-dependent protease ClpAP ELISA KIT ,Porcine proteolytic subunit ELISA KIT , Porcine endopeptidase Clp ELISA KIT ,Porcine caseinolytic mitochondrial matrix peptidase proteolytic subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine putative atp-dependent clp protease proteolytic subunit, mitochondrial,CLPP concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CLPP, During the reaction, CLPP in the sample or standard competes with a fixed amount of biotin-labeled CLPP for sites on a pre-coated Monoclonal antibody specific to CLPP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CLPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
