Porcine COLLAGEN TYPE II ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  Col II/ COL2A1

Protein Name  Collagen Type II

Alternative Name

Collagen Type II,type II collagen,collagen type II alpha 1 chain; ANFH; AOM; COL11A3; SEDC; STL1; collagen alpha-1(II) chain; alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; chondrocalcin; collagen II, alpha-1 polypeptide; collagen, type II, alpha 1

Intended use

Porcine COLLAGEN TYPE II ELISA KIT allows for the in vitro quantitative determination of COLLAGEN TYPE II concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Porcine COLLAGEN TYPE II ELISA KIT has been pre-coated with an COLLAGEN TYPE II antibody specific to COLLAGEN TYPE II. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for COLLAGEN TYPE II and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain COLLAGEN TYPE II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of COLLAGEN TYPE II in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20