Porcine Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ENTPD1, Class 3.6.1.5,Ectonucleoside triphosphate diphosphohydrolase 1,NTPDase 1,Lymphoid cell activation antigen,Ecto-ATP diphosphohydrolase 1,Ecto-ATPDase 1,Ecto-ATPase 1,Ecto-apyrase, CD39,NTPDase 1; ATPDase; NTPDase-1; SPG64; CD39 antigen; ecto-ATP diphosphohydrolase 1; ecto-ATPDase 1; ecto-ATPase 1; ecto-apyrase; lymphoid cell activation antigen; ectonucleoside triphosphate diphosphohydrolase 1
Search name
Porcine ENTPD1 ELISA KIT ,Porcine Class 3.6.1.5 ELISA KIT ,Porcine Ectonucleoside triphosphate diphosphohydrolase 1 ELISA KIT ,Porcine NTPDase 1 ELISA KIT ,Porcine Lymphoid cell activation antigen ELISA KIT ,Porcine Ecto-ATP diphosphohydrolase 1 ELISA KIT ,Porcine Ecto-ATPDase 1 ELISA KIT ,Porcine Ecto-ATPase 1 ELISA KIT ,Porcine Ecto-apyrase ELISA KIT , Porcine CD39 ELISA KIT ,Porcine NTPDase 1 ELISA KIT ,Porcine ATPDase ELISA KIT ,Porcine NTPDase-1 ELISA KIT ,Porcine SPG64 ELISA KIT ,Porcine CD39 antigen ELISA KIT ,Porcine ecto-ATP diphosphohydrolase 1 ELISA KIT ,Porcine ecto-ATPDase 1 ELISA KIT ,Porcine ecto-ATPase 1 ELISA KIT ,Porcine ecto-apyrase ELISA KIT ,Porcine lymphoid cell activation antigen ELISA KIT ,Porcine ectonucleoside triphosphate diphosphohydrolase 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine ectonucleoside triphosphate diphosphohydrolase 1,NTPDase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NTPDase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NTPDase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NTPDase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NTPDase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.