Porcine Glutamine synthetase, GS ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Glutamine synthetase,GS,Glutamate decarboxylase,Glutamate--ammonia ligase,Glutamine synthetase,GLNS; PIG43; PIG59; cell proliferation-inducing protein 59; glutamate decarboxylase; glutamate--ammonia ligase; glutamine synthase; proliferation-inducing protein 43; glutamate-ammonia ligase,GLUL

Search name

Porcine Glutamine synthetase ELISA KIT ,Porcine GS ELISA KIT ,Porcine GLUL ELISA KIT, Porcine GLNS ELISA KIT ,Porcine PIG43 ELISA KIT ,Porcine PIG59 ELISA KIT ,Porcine cell proliferation-inducing protein 59 ELISA KIT ,Porcine glutamate decarboxylase ELISA KIT ,Porcine glutamate--ammonia ligase ELISA KIT,Porcine Glutamate decarboxylase ELISA KIT ,Porcine Glutamate--ammonia ligase ELISA KIT ,Porcine Glutamine synthetase ELISA KIT ,Porcine GLNS ELISA KIT ,Porcine PIG43 ELISA KIT ,Porcine PIG59 ELISA KIT ,Porcine cell proliferation-inducing protein 59 ELISA KIT ,Porcine glutamate decarboxylase ELISA KIT ,Porcine glutamate--ammonia ligase ELISA KIT ,Porcine glutamine synthase ELISA KIT ,Porcine proliferation-inducing protein 43 ELISA KIT ,Porcine glutamate-ammonia ligase ELISA KIT,Porcine GLUL ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine glutamine synthetase,GS concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to GS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GS in the samples is then determined by comparing the O.D. of the samples to the standard curve.