Porcine GM-CSF ELISA Kit

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  • Model: ELA-E0045p
  • 20 Units in Stock
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Porcine Granulocyte macrophage colony stimulating factor, GM- CSF ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Granulocyte-macrophage colony-stimulating factor,GM-CSF,Colony-stimulating factor,CSF, CSF2,GM-CSF,GMCSF; CSF; granulocyte-macrophage colony-stimulating factor; molgramostin; sargramostim; colony stimulating factor 2,granulocyte-macrophage

 

Search name

Porcine Granulocyte-macrophage colony-stimulating factor ELISA KIT ,Porcine GM-CSF ELISA KIT ,Porcine Colony-stimulating factor ELISA KIT ,Porcine CSF ELISA KIT ,Porcine CSF2 ELISA KIT ,Porcine GM-CSF ELISA KIT ,Porcine GMCSF ELISA KIT ,Porcine CSF ELISA KIT ,Porcine granulocyte-macrophage colony-stimulating factor ELISA KIT ,Porcine molgramostin ELISA KIT ,Porcine sargramostim ELISA KIT ,Porcine colony stimulating factor 2 ELISA KIT ,Porcine granulocyte-macrophage ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine granulocyte-macrophage colony-stimulating factor,GM-CSF concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GM-CSF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GM-CSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GM-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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