Porcine Interleukin- 6, IL- 6 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Interleukin-6,IL-6, IL-6,BSF2; HGF; HSF; IFNB2; B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation
factor; IFN-beta-2; hybridoma growth factor; interferon beta-2; interleukin BSF-2; interleukin-6; interleukin 6 (interferon, beta 2)
Search name
Porcine Interleukin- 6 ELISA Kit, Porcine IL-6 ELISA Kit, Porcine Interleukin6 ELISA Kit, Porcine IL6 ELISA Kit, Interleukin- 6
ELISA Kit, IL-6 ELISA Kit, Interleukin6 ELISA Kit, IL6 ELISA Kit,Porcine BSF2 ELISA Kit, Porcine HGF ELISA Kit, Porcine HSF
ELISA Kit, Porcine IFNB2 ELISA Kit, Porcine BSF-2 ELISA Kit, Porcine CDF ELISA Kit,BSF2 ELISA Kit, HGF ELISA Kit, HSF
ELISA Kit, IFNB2 ELISA Kit, BSF-2 ELISA Kit, CDF ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine interleukin-6,IL-6 concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-6. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-6 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain IL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-6 in the samples is then determined by comparing the
O.D. of the samples to the standard curve.
PDF manual download