Porcine Interferon- stimulated gene 20 kDa protein, ISG20 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ISG20,promyelocytic leukemia nuclear body-associated protein ISG20; CD25; HEM45; estrogen-regulated transcript 45 protein; interferon-stimulated gene 20 kDa protein; interferon stimulated exonuclease gene 20kDa

Search name

Porcine ISG20 ELISA KIT ,Porcine promyelocytic leukemia nuclear body-associated protein ISG20 ELISA KIT ,Porcine CD25 ELISA KIT ,Porcine HEM45 ELISA KIT ,Porcine estrogen-regulated transcript 45 protein ELISA KIT ,Porcine interferon-stimulated gene 20 kDa protein ELISA KIT ,Porcine interferon stimulated exonuclease gene 20kDa ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine Interferon- stimulated gene 20 kDa protein, ISG20 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ISG20. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ISG20 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ISG20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ISG20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.