Porcine Matrix metalloproteinase 2,MMP2 ELISA Kit

Starting at: $796.00

  • Model: ELA-E0100p
  • 20 Units in Stock
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Porcine Matrix metalloproteinase 2, MMP2 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

MMP2,MMP-2,CLG4; CLG4A; MMP-II; MONA; TBE-1; 72 kDa gelatinase; 72 kDa type IV collagenase; collagenase type IV-A; gelatinase A; matrix metalloproteinase-2; matrix metalloproteinase-II; neutrophil gelatinase; matrix metallopeptidase 2 gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase

 

Search name

Porcine MMP2 ELISA KIT ,Porcine MMP-2 ELISA KIT ,Porcine CLG4 ELISA KIT ,Porcine CLG4A ELISA KIT ,Porcine MMP-II ELISA KIT ,Porcine MONA ELISA KIT ,Porcine TBE-1 ELISA KIT ,Porcine 72 kDa gelatinase ELISA KIT ,Porcine 72 kDa type IV collagenase ELISA KIT ,Porcine collagenase type IV-A ELISA KIT ,Porcine gelatinase A ELISA KIT ,Porcine matrix metalloproteinase-2 ELISA KIT ,Porcine matrix metalloproteinase-II ELISA KIT ,Porcine neutrophil gelatinase ELISA KIT ,Porcine matrix metallopeptidase 2 gelatinase A ELISA KIT ,Porcine 72kDa gelatinase ELISA KIT ,Porcine 72kDa type IV collagenase ELISA KIT 

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine 72 kDa type IV collagenase concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to 72 kDa type IV collagenase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 72 kDa type IV collagenase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 72 kDa type IV collagenase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 72 kDa type IV collagenase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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