Porcine matrix metalloproteinase 9/ Gelatinase B, MMP9 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Matrix metalloproteinase-9(MMP-9),Gelatinase B(GELB),92 kDa gelatinase,92 kDa type IV collagenase, MMP-9,matrix metallopeptidase
9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type
IV collagenase); CLG4B; GELB; MANDP2; 92 kDa gelatinase; 92 kDa type IV collagenase; macrophage gelatinase; matrix
metalloproteinase-9; type V collagenase

Search name
Porcine matrix metalloproteinase 9 ELISA Kit, Porcine MMP- 9 ELISA Kit, Porcine Gelatinase B ELISA Kit, Porcine GELB ELISA Kit,
Porcine 92 kDa gelatinase ELISA Kit, Porcine 92 kDa type IV collagenas ELISA Kit, Porcine CLG4B ELISA Kit, Porcine MANDP2
ELISA Kit, Porcine macrophage gelatinase ELISA Kit, Porcine type V collagenase ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine matrix metalloproteinase-9,MMP-9 concentrations in
serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-9. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MMP-9 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain MMP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MMP-9 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.