Porcine Metallothionein- 2, MT2A ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MT2A,MT2; MT-2; MT-II; Metallothionein-2,metallothionein-2A; metallothionein-II; metallothionein 2A

Search name

Porcine MT2A ELISA KIT ,Porcine MT2 ELISA KIT ,Porcine MT-2 ELISA KIT ,Porcine MT-II ELISA KIT ,Porcine Metallothionein-2 ELISA KIT ,Porcine metallothionein-2A ELISA KIT ,Porcine metallothionein-II ELISA KIT ,Porcine metallothionein 2A ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of porcine metallothionein-2,MT-2 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MT-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MT-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MT-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MT-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.