Porcine Nucleoside diphosphate kinase B, NME2 ELISA KIT

Product Name:Porcine Nucleoside diphosphate kinase B, NME2 ELISA KIT
Packing:96T

Catalog No.:ELI-23030p

Gene Name:NME2

Detect Range:0.78-50 ng/mL

Sensitivity:0.24ng/mL

Target Protein Name:Porcine Nucleoside diphosphate kinase B

Alternative Name:NME2,Porcine Nucleoside diphosphate kinase B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Porcine Nucleoside diphosphate kinase B, NME2 ELISA KIT allows for the in vitro quantitative determination of Porcine Nucleoside diphosphate kinase B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Porcine Nucleoside diphosphate kinase B, NME2 ELISA KIT has been pre-coated with an NME2 antibody specific to Porcine Nucleoside diphosphate kinase B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Porcine Nucleoside diphosphate kinase B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Porcine Nucleoside diphosphate kinase B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Porcine Nucleoside diphosphate kinase B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!