Porcine Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial, SDHA ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

SDHA,CMD1GG; PGL5; SDH1; SDH2; SDHF; flavoprotein subunit of complex II; succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; succinate dehydrogenase complex flavoprotein subunit; succinate dehydrogenase complex, subunit A, flavoprotein (Fp),Flavoprotein subunit of complex II

Search name

Porcine SDHA ELISA KIT ,Porcine CMD1GG ELISA KIT ,Porcine PGL5 ELISA KIT ,Porcine SDH1 ELISA KIT ,Porcine SDH2 ELISA KIT ,Porcine SDHF ELISA KIT ,Porcine flavoprotein subunit of complex II ELISA KIT ,Porcine succinate dehydrogenase [ubiquinone] flavoprotein subunit ELISA KIT ,Porcine  mitochondrial ELISA KIT ,Porcine succinate dehydrogenase complex flavoprotein subunit ELISA KIT ,Porcine succinate dehydrogenase complex ELISA KIT ,Porcine  subunit A ELISA KIT ,Porcine  flavoprotein ELISA KIT ,Porcine Fp ELISA KIT ,Porcine Flavoprotein subunit of complex II ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of SDHA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDHA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SDHA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SDHA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SDHA in the samples is then determined by comparing the O.D. of the samples to the standard curve.