Porcine Sex determining region Y protein, SRY ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Sex-determining region Y protein,Testis-determining factor, SRY,SRXX1; SRXY1; TDF; TDY; essential protein for sex determination in Porcine males; sex-determining region Y protein; sex-determining region on Y; testis-determining factor on Y; sex determining region Y
Search name
Porcine Sex-determining region Y protein ELISA KIT ,Porcine Testis-determining factor ELISA KIT ,Porcine SRY ELISA KIT ,Porcine SRXX1 ELISA KIT ,Porcine SRXY1 ELISA KIT ,Porcine TDF ELISA KIT ,Porcine TDY ELISA KIT ,Porcine essential protein for sex determination in Porcine males ELISA KIT ,Porcine sex-determining region Y protein ELISA KIT ,Porcine sex-determining region on Y ELISA KIT ,Porcine testis-determining factor on Y ELISA KIT ,Porcine sex determining region Y ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine sex-determining region y protein,SRY concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SRY. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SRY and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SRY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SRY in the samples is then determined by comparing the O.D. of the samples to the standard curve.