Porcine Soluble Intercellular Adhesion Molecule- 1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Soluble Intercellular adhesion molecule 1(sICAM-1), Soluble Major group rhinovirus receptor, sICAM, Soluble CD54; Soluble rhinovirus
receptor; sBB2; sP3.58; Soluble cell surface glycoprotein P3.58; MALA-2,MyD10,;
Search name
Porcine sICAM-1 ELISA Kit, Porcine Soluble Intercellular adhesion molecule 1 ELISA Kit, Porcine Soluble Major group rhinovirus
receptor ELISA Kit, Porcine sICAM ELISA Kit, Porcine Soluble CD54 ELISA Kit, Porcine Soluble rhinovirus receptor ELISA Kit,
Porcine sBB2 ELISA Kit, Porcine sP3.58 ELISA Kit, Porcine Soluble cell surface glycoprotein P3.58 ELISA Kit, Porcine Soluble
MALA-2 ELISA Kit, Porcine Soluble MyD10 ELISA Kit
Intended use
Porcine Soluble Intercellular Adhesion Molecule- 1 ELISA Kit allows for the in vitro quantitative determination of Porcine SICAM-1 concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Porcine Soluble Intercellular Adhesion Molecule- 1 ELISA Kithas been pre-coated with an antibody specific to SICAM-1. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SICAM-1 and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain SICAM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SICAM-1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
Porcine Soluble Intercellular Adhesion Molecule- 1 ELISA Kit Manual download