Porcine Signal transducer and activator of transcription 3, STAT3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Signal transducer and activator of transcription 3,Acute-phase response factor, STAT3,APRF; HIES; DNA-binding protein APRF; acute-phase response factor; signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 ,acute-phase response factor,

Search name

Porcine STAT3 ELISA KIT ,Porcine Signal transducer and activator of transcription 3 ELISA KIT ,Porcine Acute-phase response factor ELISA KIT ,Porcine APRF ELISA KIT ,Porcine HIES ELISA KIT ,Porcine DNA-binding protein APRF ELISA KIT ,Porcine acute-phase response factor ELISA KIT ,Porcine signal transducer and activator of transcription 3 ELISA KIT ,Porcine signal transducer and activator of transcription 3  ELISA KIT ,Porcine acute-phase response factor ELISA KIT

Intended use

Porcine STAT3 ELISA KIT allows for the in vitro quantitative determination of Porcine Signal transducer and activator of transcription 3, STAT3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Porcine STAT3 ELISA KIT has been pre-coated with an antibody specific to STAT3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for STAT3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain STAT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of STAT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Porcine STAT3 ELISA KIT Range 0.15-10ng/ml

Porcine STAT3 ELISA KIT PDF Manual download

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