Porcine Troponin C, slow skeletal and cardiac muscles, TNNC1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TNNC1,CMD1Z; CMH13; TN-C; TNC; TNNC; cardiac troponin C; slow twitch skeletal/cardiac muscle troponin C; troponin C type 1 (slow); troponin C1, slow; troponin C1, slow skeletal and cardiac type

Search name

Porcine TNNC1 ELISA Kit ,Porcine CMD1Z ELISA Kit ,Porcine CMH13 ELISA Kit ,Porcine TN-C ELISA Kit ,Porcine TNC ELISA Kit ,Porcine TNNC ELISA Kit ,Porcine cardiac troponin C ELISA Kit ,Porcine slow twitch skeletal/cardiac muscle troponin C ELISA Kit ,Porcine troponin C type 1 (slow) ELISA Kit ,Porcine troponin C1 slow ELISA Kit ,Porcine troponin C1 slow skeletal and cardiac type ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine Troponin C, slow skeletal and cardiac muscles, TNNC1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Porcine Troponin C,slow skeletal and cardiac muscles,TNNC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Porcine Troponin C,slow skeletal and cardiac muscles,TNNC1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Porcine Troponin C,slow skeletal and cardiac muscles,TNNC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Porcine Troponin C,slow skeletal and cardiac muscles,TNNC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.