Porcine tumor necrosis factor Alpha,TNFA ELISA Kit

Starting at: $796.00

  • Model: ELA-E0133p
  • 20 Units in Stock
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Porcine Tumor necrosis factor, TNFA ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Tumor necrosis factor,Tumor necrosis factor ligand superfamily member 2,TNF-a,Cachectin,TNF-alpha, TNF-alpha,TNFA; DIF; TNFSF2; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor necrosis factor

 

Search name

Porcine Tumor necrosis factor ELISA KIT ,Porcine Tumor necrosis factor ligand superfamily member 2 ELISA KIT ,Porcine TNF-a ELISA KIT ,Porcine Cachectin ELISA KIT ,Porcine TNF-alpha ELISA KIT ,Porcine  TNF-alpha ELISA KIT ,Porcine TNFA ELISA KIT ,Porcine DIF ELISA KIT ,Porcine TNFSF2 ELISA KIT ,Porcine APC1 protein ELISA KIT ,Porcine TNF ELISA KIT ,Porcine  macrophage-derived ELISA KIT ,Porcine TNF ELISA KIT ,Porcine  monocyte-derived ELISA KIT ,Porcine TNF-a ELISA KIT ,Porcine cachectin ELISA KIT ,Porcine tumor necrosis factor ligand superfamily member 2 ELISA KIT ,Porcine tumor necrosis factor-alpha ELISA KIT ,Porcine tumor necrosis factor ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Porcine tumor necrosis factor,Tumor necrosis factor concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor necrosis factor. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tumor necrosis factor and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tumor necrosis factor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tumor necrosis factor in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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