Porcine Vascuolar cell adhesion molecule 1,VCAM-1 ELISA kit

Starting at: $796.00

  • Model: ELA-E0142p
  • 20 Units in Stock
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Porcine Vascuolar cell adhesion molecule 1, VCAM- 1 ELISA kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Vascular cell adhesion protein 1(V-CAM 1,VCAM-1),INCAM-100, VCAM-1,CD106; INCAM-100; CD106 antigen; vascular cell adhesion
protein 1; vascular cell adhesion molecule 1

Search name
Porcine Vascular cell adhesion protein 1 ELISA Kit, Porcine V-CAM 1 ELISA Kit, Porcine Vascuolar cell adhesion molecule 1 ELISA
kit, Porcine VCAM-1 ELISA kit, Porcine INCAM-100 ELISA kit, Porcine CD106 ELISA kit, Porcine CD106 antigen ELISA kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine vascular cell adhesion protein 1,V-CAM 1 concentrations
in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-CAM 1. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for V-CAM 1 and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain V-CAM 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of V-CAM 1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

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