Rabbit Glial fibrillary acidic protein, GFAP ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms
Glial fibrillary acidic protein(GFAP), FLJ42474; FLJ45472

Search name
Rabbit Glial fibrillary acidic protein ELISA Kit, Rabbit GFAP ELISA Kit, Rabbit FLJ42474 ELISA Kit, Rabbit FLJ45472 ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rabbit glial fibrillary acidic protein,GFAP concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GFAP. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GFAP and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain GFAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GFAP in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

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