Rabbit Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, HCN4 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4,HCN4,SSS2,hyperpolarization activated cyclic nucleotide-gated cation channel 4, Bcng3,Brain cyclic nucleotide-gated channel 3,BCNG-3,HAC4,HAC-4
Search name
Rabbit Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 ELISA KIT ,Rabbit HCN4 ELISA KIT ,Rabbit SSS2 ELISA KIT ,Rabbit hyperpolarization activated cyclic nucleotide-gated cation channel 4 ELISA KIT ,Rabbit Bcng3 ELISA KIT ,Rabbit Brain cyclic nucleotide-gated channel 3 ELISA KIT ,Rabbit BCNG-3 ELISA KIT ,Rabbit HAC4 ELISA KIT ,Rabbit HAC-4 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rabbit potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4,HCN4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HCN4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HCN4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HCN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HCN4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
