Rat 25- OH Vitamin D ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
25-OH Vitamin D,

Search name
Rat 25-OH Vitamin D ELISA Kit, 25-OH Vitamin D ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of 25-oh vitamin d,25-OH Vitamin D concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to 25-OH Vitamin D, During the reaction, 25-OH Vitamin D in the sample or standard competes with a
fixed amount of biotin-labeled 25-OH Vitamin D for sites on a pre-coated Monoclonal antibody specific to 25-OH Vitamin D. Excess
conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is
added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of 25-OH Vitamin D in the samples is then determined by comparing the O.D. of the samples to the
standard curve.

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