Rat 3- Nitrotyrosine,3- NT ELISA Kit

Starting at: $756.00

  • Model: ELA-E1863r
  • 20 Units in Stock
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Rat 3-NT ELISA Kit

96 Tests

Operating instructions

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Nitrotyrosine, NT, 3-Nitrotyrosine, 3-Nitro-L-tyrosine, 3NT

Search name

Rat Nitrotyrosine ELISA KIT ,Rat NT ELISA KIT ,Rat 3-Nitrotyrosine ELISA KIT ,Rat 3-Nitro-L-tyrosine ELISA KIT ,Rat 3NT ELISA KIT

 

Intended use

Rat 3-NT ELISA Kit allows for the in vitro quantitative determination of 3-NT concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

Rat 3-NT ELISA Kit is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in Rat 3-NT ELISA Kit has been pre-coated with an antibody specific to 3-NT, During the reaction, 3-NT in the sample or standard competes with a fixed amount of biotin-labeled 3-NT for sites on a pre-coated Monoclonal antibody specific to 3-NT. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 3-NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Rat 3-NT ELISA Kit Range 15.6-1000pg/mL

 

3-NT Related Products

Rat 3- Nitrotyrosine,3- NT ELISA Kit

Rat 3- Nitrotyrosine,3- NT ELISA Kit

 

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