Rat 6- phosphofructokinase, muscle type, Pfkm ELISA Kit
FOR RESEARCH USE ONLY!

96 Tests

Alternative name

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Intended use

Rat Pfkm ELISA kit allows for the in vitro quantitative determination of Rat Pfkm concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Rat Pfkm ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pfkm and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pfkm, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pfkm in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Rat Pfkm ELISA kit Range 0.31-20ng/ml

Rat Pfkm ELISA kit PDF manual download

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