Rat Acid ceramidase,Asah1 ELISA KIT

Starting at: $756.00

  • Model: ELI-49149r
  • 20 Units in Stock
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Rat Acid ceramidase,ASAH1 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

ASAH1,ACDase; ASAH; PHP; PHP32; SMAPME; acid CDase; acid ceramidase; acylsphingosine deacylase; N-acylsphingosine amidohydrolase (acid ceramidase) 1

 

Search name

Rat ASAH1 ELISA KIT ,Rat ACDase ELISA KIT ,Rat ASAH ELISA KIT ,Rat PHP ELISA KIT ,Rat PHP32 ELISA KIT ,Rat SMAPME ELISA KIT ,Rat acid CDase ELISA KIT ,Rat acid ceramidase ELISA KIT ,Rat acylsphingosine deacylase ELISA KIT ,Rat N-acylsphingosine amidohydrolase 1 ELISA KIT ,Rat N-acylsphingosine acid ceramidase 1 ELISA KIT

 

Intended use

Rat ASAH1 ELISA KIT allows for the in vitro quantitative determination of Rat Acid ceramidase,ASAH1 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in Rat ASAH1 ELISA KIT has been pre-coated with an antibody specific to Rat Acid ceramidase,ASAH1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rat Acid ceramidase,ASAH1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Acid ceramidase,ASAH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Acid ceramidase,ASAH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Rat ASAH1 ELISA KIT manual request by email:sales@lifescience-market.com  

Order ASAH1 ELISA KIT by email:sales@lifescience-market.com

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