Rat Adiponectin,ADIPOQ ELISA KIT

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  • Model: ELA-E0605r
  • 20 Units in Stock
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Rat Adiponectin, ADIPOQ ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

ADIPOQ,adiponectin, Adipocyte,ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; gelatin-binding protein 28; adiponectin, C1Q and collagen domain containing

 

Search name

Rat ADIPOQ ELISA KIT ,Rat adiponectin ELISA KIT ,Rat Adipocyte ELISA KIT ,Rat ACDC ELISA KIT ,Rat ACRP30 ELISA KIT ,Rat ADIPQTL1 ELISA KIT ,Rat ADPN ELISA KIT ,Rat APM-1 ELISA KIT ,Rat APM1 ELISA KIT ,Rat GBP28 ELISA KIT ,Rat 30 kDa adipocyte complement-related protein ELISA KIT ,Rat adipocyte complement-related 30 kDa protein ELISA KIT ,Rat adipose most abundant gene transcript 1 protein ELISA KIT ,Rat adipose specific collagen-like factor ELISA KIT ,Rat gelatin-binding protein 28 ELISA KIT ,Rat adiponectin ELISA KIT ,Rat C1Q and collagen domain containing ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Adiponectin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Adiponectin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Adiponectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Adiponectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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