Rat ADP-ribose pyrophosphatase mitochondrial,Nudt9 ELISA KIT

Rat ADP- ribose pyrophosphatase mitochondrial, NUDT9 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

NUDT9,NUDT10,PSEC0099,Nucleoside diphosphate-linked moiety X motif 9,Nudix motif 9;Adenosine diphosphoribose pyrophosphatase,ADPR-PPase;ADP-ribose diphosphatase;ADP-ribose phosphohydrolase,ADP-ribose pyrosphosphatase NUDT9; ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ADP-ribose pyrophosphatase mitochondrial; nudix (nucleoside diphosphate linked moiety X)-type motif 9

Search name

Rat NUDT9 ELISA KIT ,Rat NUDT10 ELISA KIT ,Rat PSEC0099 ELISA KIT ,Rat Nucleoside diphosphate-linked moiety X motif 9 ELISA KIT ,Rat Nudix motif 9 ELISA KIT ,Rat Adenosine diphosphoribose pyrophosphatase ELISA KIT ,Rat ADPR-PPase ELISA KIT ,Rat ADP-ribose diphosphatase ELISA KIT ,Rat ADP-ribose phosphohydrolase ELISA KIT ,Rat ADP-ribose pyrosphosphatase NUDT9 ELISA KIT ,Rat ADP-ribose diphosphatase ELISA KIT ,Rat ADP-ribose phosphohydrolase ELISA KIT ,Rat ADP-ribose pyrophosphatase mitochondrial ELISA KIT ,Rat nudix (nucleoside diphosphate linked moiety X)-type motif 9 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat ADP- ribose pyrophosphatase, mitochondrial, NUDT9 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADP- ribose pyrophosphatase, mitochondrial, NUDT9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rat ADP- ribose pyrophosphatase, mitochondrial, NUDT9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat ADP- ribose pyrophosphatase, mitochondrial, NUDT9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat ADP- ribose pyrophosphatase, mitochondrial, NUDT9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.