Rat advanced glycation end products, AGEs ELISA Kit

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  • Model: ELA-E0263r
  • 20 Units in Stock
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Rat advanced glycation end products, AGEs ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

 

Alternative name

Rat AGES ELISA KIT ,Rat AGE ELISA KIT ,Rat Advanced glycation end-products ELISA KIT ,Rat Advanced glycation end product ELISA KIT

 

Intended use

Rat AGES ELISA KIT allows for the in vitro quantitative determination of rat Advanced glycation end-product concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

Rat AGES ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Advanced glycation end-product, During the reaction, Advanced glycation end-product in the sample or standard competes with a fixed amount of biotin-labeled Advanced glycation end-product for sites on a pre-coated Monoclonal antibody specific to Advanced glycation end-product. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Advanced glycation end-product in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.78-50ng/ml

Rat AGES ELISA KIT Manual download

Order Rat AGES ELISA KIT by email:sales@lifescience-market.com
 

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