Rat AKT- interacting protein, AKTIP ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

AKTIP,FT1; FTS; fused toes homolog; AKT interacting protein

Search name

Rat AKTIP ELISA KIT ,Rat FT1 ELISA KIT ,Rat FTS ELISA KIT ,Rat fused toes homolog ELISA KIT ,Rat AKT interacting protein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination ofRat AKT-interacting proteinconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to AKT-interacting protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forAKT-interacting protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AKT-interacting protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AKT-interacting protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.