Rat Muellerian-inhibiting factor, AMH ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Muellerian-inhibiting factor,Anti-Muellerian hormone(AMH),Muellerian-inhibiting substance(MIS), MIF,MIS,Mullerian inhibiting
factor,Mullerian inhibiting substance,anti-Muellerian hormone,muellerian-inhibiting factor,muellerian-inhibiting
substance,anti-Mullerian hormone

Search name
Rat Muellerian-inhibiting factor ELISA Kit, Rat AMH ELISA Kit,Muellerian-inhibiting factor ELISA Kit,AMH ELISA Kit,
Muellerian-inhibiting factor,Anti-Muellerian hormone(AMH),Muellerian-inhibiting substance(MIS), MIF,MIS,Mullerian
inhibiting factor,Mullerian inhibiting substance,anti-Muellerian hormone,muellerian-inhibiting factor,muellerian-inhibiting
substance,anti-Mullerian hormone, ELISA KIT

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Muellerian-inhibiting factor concentrations in serum, Plasma,
Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Muellerian-inhibiting factor. Standards or samples
are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for
Muellerian-inhibiting factor and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then
a TMB substrate solution is added to each well. Only those wells that contain Muellerian-inhibiting factor, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Muellerian-inhibiting factor in the samples is then determined by comparing the O.D. of the samples to the standard curve.