Rat Apelin- 36 , AP36 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms
Apelin-36, APLN,apelin,APEL; XNPEP2; AGTRL1 ligand; APJ endogenous ligand, APLN36

Search name
Rat Apelin-36 ELISA Kit,Apelin-36 ELISA Kit, APLN36 ELISA Kit, Rat APLN ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat apelin-36,Apelin-36 concentrations in serum, Plasma,
Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apelin-36. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apelin-36 and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Apelin-36, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Apelin-36 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.