Rat Adipocyte plasma membrane associated protein, APMAP ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Adipocyte plasma membrane-associated protein,Protein BSCv, BSCv,C20orf3; adipocyte plasma membrane-associated protein; protein
BSCv; adipocyte plasma membrane associated protein

Search name
Rat Adipocyte plasma membrane associated protein ELISA Kit, Rat APMAP ELISA Kit, Rat Adipocyte plasma
membrane-associated protein ELISA Kit, Rat Protein BSCv ELISA Kit, Rat BSCv ELISA Kit, Rat C20orf3 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat adipocyte plasma membrane-associated protein,APMAP
concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to APMAP. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for APMAP and Avidin conjugated
to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain APMAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of APMAP in the samples is then determined by comparing
the O.D. of the samples to the standard curve.