Rat Apold1 ELISA Kit

$566.00

  • Model: EF018336
  • 100 Units in Stock
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Rat Apold1 ELISA Kit

Catalog   EF018336                                                                         Manual

Packing 48Tests/96Tests

ELISA Method   Sandwich ELISA     Species  Rat

Sample   Rat serum, plasma, tissue homogenates and other biological fluids and Recombinant Apold1

Detect Range 0.156-10ng/ml   sensitivity  0.094ng/ml


ELISA Intend Use

Rat Apold1 ELISA Kit is a Sandwich ELISA KIT detecting the concentration of Apolipoprotein L domain-containing protein 1 in Rat serum, plasma, tissue homogenates and other biological fluids.
 

 

【INTRODUCTION】
APOLD1 is an endothelial cell early response protein that may play a role in regulation of endothelial cell signaling and vascular function. The deduced human APOLD1 protein contains 348 amino acids and shares 87% identity with the 246-amino acid rodent Apold1 proteins. APOLD1 contains a predicted N-terminal leucine zipper motif, 2 hydrophobic regions, a C-terminal coiled-coil 4 domain, and several predicted phosphorylation sites. In situ analysis detected high levels of Apold1 mRNA in the rat embryonic heart and decreased levels in adult heart with expression restricted to capillaries and the inner surfaces of arteries, and veins. Dual fluorescent cell labeling with an endothelial cell marker demonstrated endothelial cell localization of Apold1 in vessels of all rat tissues sampled.

 

【PRINCIPLE OF THE ASSAY】
This assay employs a two-site sandwich ELISA to quantitate APOLD1 in Rat serum, plasma. An antibody specific for APOLD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOLD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOLD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOLD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

 

【LIMITATIONS OF THE PROCEDURE】
※ FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
※ The kit should not be used beyond the expiration date on the kit label.
※ Do not mix or substitute reagents with those from other lots or sources.
※ It is important that the Calibrator Diluent selected for the standard curve be
consistent with the samples being assayed.
※    If samples generate values higher than the highest standard, dilute the samples with the appropriate Calibrator Diluent and repeat the assay.
※    Any variation in standard diluent, operator, pipetting technique, washing
technique, incubation time or temperature, and kit age can cause variation in binding.
※    This assay is designed to eliminate interference by soluble receptors,
binding proteins, and other factors present in biological samples. Until all factors have been tested in the ELISA Kit, the possibility of interference cannot be excluded.


【DETECTION RANGE】
0.156 ng/mL - 10 ng/mL. The standard curve concentrations used for the ELISA’s were 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.31 ng/mL,
0.156 ng/mL, 0 ng/mL.

【SENSITIVITY】
The limit of detection of Rat APOLD1 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.1 ng/mL (mean of 6 independent assays).

【SPECIFICITY】
This assay has high sensitivity and excellent specificity for detection of Rat APOLD1. No significant cross-reactivity or interference between Rat APOLD1 and analogues was observed.
Note:
Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Rat APOLD1 and all the analogues, therefore, cross reaction may still exist.

【PRECISION】
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)


【STABILITY】
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2-8°C, which means 7 days at 37°C equaling 12 months at 2-8°C).
Note:
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 

(ELISA KIT is only for Research Only, not for the diagnostic Use!)

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