Rat Apolipoprotein B-100,Apob ELISA KIT

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  • Model: ELI-06441r
  • 20 Units in Stock
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Rat Apolipoprotein B- 100, APOB ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

ApoB,apolipoprotein B,apoB-48; apoB-100; FLDB; LDLCQ4; apo B-100; apolipoprotein B (including Ag(x) antigen); apolipoprotein B-100; apolipoprotein B48; mutant Apo B 100

 

Search name

Rat ApoB ELISA KIT ,Rat apolipoprotein B ELISA KIT ,Rat apoB-48 ELISA KIT ,Rat apoB-100 ELISA KIT ,Rat FLDB ELISA KIT ,Rat LDLCQ4 ELISA KIT ,Rat apo B-100 ELISA KIT ,Rat apolipoprotein B (including Ag(x) antigen) ELISA KIT ,Rat apolipoprotein B-100 ELISA KIT ,Rat apolipoprotein B48 ELISA KIT ,Rat mutant Apo B 100 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat apolipoprotein b-100,Apo B-100 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apo B-100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apo B-100 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apo B-100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Apo B-100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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