Rat Aspartate aminotransferase, GOT/ ASAT ELISA Kit
96 Tests
FOR RESEARCH USE ONLY!
Alternative name
Rat ASAT ELISA KIT ,Rat Aspartate aminotransferase ELISA KIT ,Rat GOT1 ELISA KIT ,Rat GOT ELISA KIT ,Rat aspartate aminotransferase 1 ELISA KIT ,Rat AST1 ELISA KIT ,Rat ASTQTL1 ELISA KIT ,Rat GIG18 ELISA KIT ,Rat cAspAT ELISA KIT ,Rat cCAT ELISA KIT ,Rat aspartate transaminase 1 ELISA KIT ,Rat cysteine aminotransferase ELISA KIT ,Rat cytoplasmic ELISA KIT ,Rat cysteine transaminase cytoplasmic ELISA KIT ,Rat glutamate oxaloacetate transaminase 1 ELISA KIT ,Rat growth-inhibiting protein 18 ELISA KIT ,Rat transaminase A ELISA KIT ,Rat soluble glutamic-oxaloacetic transaminase 1 ELISA KIT ,Rat Aspartate aminotransferase cytoplasmic ELISA KIT
Intended use
Rat ASAT ELISA KIT allows for the in vitro quantitative determination of Rat Aspartate aminotransferase cytoplasmic concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate aminotransferase, cytoplasmic. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Aspartate aminotransferase, cytoplasmic and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Aspartate aminotransferase, cytoplasmic, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Aspartate aminotransferase, cytoplasmic in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 3.12-200U/L
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