Rat basic fibroblast growth factor ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Rat BFGF ELISA KIT ,Rat basic fibroblast growth factor ELISA KIT ,Rat FGF-2 ELISA KIT ,Rat FGF2 ELISA KIT ,Rat basic fibroblast growth factor bFGF ELISA KIT ,Rat FGFB ELISA KIT ,Rat HBGF-2 ELISA KIT ,Rat fibroblast growth factor 2 (basic) ELISA KIT ,Rat heparin-binding growth factor 2 ELISA KIT ,Rat prostatropin ELISA KIT ,Rat fibroblast growth factor 2 ELISA KIT ,Rat Heparin-binding growth factor 2 ELISA KIT ,Rat HBGF-2 ELISA KIT

Intended use

Rat BFGF ELISA KIT allows for the in vitro quantitative determination of Rat basic fibroblast growth factor concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Rat BFGF ELISA KIT has been pre-coated with an antibody specific to HBGF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HBGF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HBGF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HBGF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Rat bFGF ELISA Kit Manual download

Range 15.6-1000 pg/ml

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