Rat Beclin-1, Becn1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Beclin-1,Coiled-coil myosin-like BCL2-interacting protein,Protein GT197,ATG6,GT197, BECN1,ATG6; VPS30; beclin1; ATG6 autophagy related 6 homolog; beclin 1,coiled-coil moesin-like BCL2 interacting protein; beclin 1,coiled-coil, moesin-like BCL2-interacting protein,beclin-1; coiled-coil myosin-like BCL2-interacting protein; beclin 1 autophagy related
Search name
Rat Beclin-1 ELISA KIT ,Rat Coiled-coil myosin-like BCL2-interacting protein ELISA KIT ,Rat Protein GT197 ELISA KIT ,Rat ATG6 ELISA KIT ,Rat GT197 ELISA KIT ,Rat BECN1 ELISA KIT ,Rat ATG6 ELISA KIT ,Rat VPS30 ELISA KIT ,Rat beclin1 ELISA KIT ,Rat ATG6 autophagy related 6 homolog ELISA KIT ,Rat beclin 1 ELISA KIT ,Rat coiled-coil moesin-like BCL2 interacting protein ELISA KIT ,Rat beclin 1 ELISA KIT ,Rat coiled-coil ELISA KIT ,Rat moesin-like BCL2-interacting protein ELISA KIT ,Rat beclin-1 ELISA KIT ,Rat coiled-coil myosin-like BCL2-interacting protein ELISA KIT ,Rat beclin 1 autophagy related ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Beclin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beclin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Beclin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Beclin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Beclin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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