Rat Beta- tubulin, TUBB ELISA kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TTUBB,CDCBM6; M40; OK/SW-cl.56; TUBB1; TUBB5; beta Ib tubulin; tubulin beta-1 chain; tubulin beta-5 chain; tubulin beta polypeptide; tubulin beta class I, Tubulin beta chain
Search name
Rat TTUBB ELISA KIT ,Rat CDCBM6 ELISA KIT ,Rat M40 ELISA KIT ,Rat OK/SW-cl.56 ELISA KIT ,Rat TUBB1 ELISA KIT ,Rat TUBB5 ELISA KIT ,Rat beta Ib tubulin ELISA KIT ,Rat tubulin beta-1 chain ELISA KIT ,Rat tubulin beta-5 chain ELISA KIT ,Rat tubulin beta polypeptide ELISA KIT ,Rat tubulin beta class I ELISA KIT ,Rat Tubulin beta chain ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Tubulin beta concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tubulin beta chain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tubulin beta chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tubulin beta chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tubulin beta chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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