Rat Calcitriol ELISA Kit
Nova Lifetech®
www.lifesciecen-market.com
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Calcitriol,1,25-dihydroxycholecalciferol,1,25-dihydroxyvitamin D3,DVD,DHVD 3
Search name
Rat Calcitriol ELISA Kit, Rat 1,25-dihydroxycholecalciferol ELISA Kit, Rat 1,25-dihydroxyvitamin D3 ELISA Kit, Rat DVD ELISA Kit, Rat DHVD 3 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Calcitriol concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcitriol, During the reaction, Calcitriol in the sample or standard competes with a fixed amount of biotin-labeled Calcitriol for sites on a pre-coated Monoclonal antibody specific to Calcitriol. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Calcitriol in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials and components
Reagent Quantity
Assay plate (Item No. CELI-66118r-1) 1
Standard (Item No. CELI-66118r-2) 2
Sample Diluent (Item No. CELI-66118r-3) 1 × 20ml
Assay Diluent A (Item No. CELI-66118r-4) 1 × 10ml
Assay Diluent B (Item No. CELI-66118r-5) 1 × 10ml
Detection Reagent A (Item No. CELI-66118r-6) 1 × 60μl
Detection Reagent B (Item No. CELI-66118r-7) 1 × 120μl
Wash Buffer(25 x concentrate) (Item No. CELI-66118r-8) 1 × 30ml
Substrate (Item No. CELI-66118r-9) 1 × 10ml
Stop Solution (Item No. CELI-66118r-10) 1 × 10ml
Plate sealer 1
Manual 1
Other supplies required
Microplate reader.
Pipettes and pipette tips.
EP tube
Deionized or distilled water.
Detection Range
15.6-1000 pg/mL.
TYPICAL DATA
This graph data is shown as an example. A standard curve must be generated each time the assay is run.
|
Concentration(pg/mL)
|
Mean OD450
|
|
1000
|
0.918
|
|
500
|
0.279
|
|
250
|
0.493
|
|
125
|
0.645
|
|
62.5
|
1.14
|
|
31.2
|
1.469
|
|
15.6
|
1.818
|
|
0
|
2.418
|
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