Rat Calcitriol ELISA Kit

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  • Model: CELI-66118r
  • 20 Units in Stock
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Rat Calcitriol ELISA Kit

Catalog No: CELI-66118r                                                     Manual download

96 Tests

Operating instructions

Nova Lifetech®

www.lifesciecen-market.com

                                                                                                             

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!

PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Calcitriol,1,25-dihydroxycholecalciferol,1,25-dihydroxyvitamin D3,DVD,DHVD 3

 

Search name

Rat Calcitriol ELISA Kit, Rat 1,25-dihydroxycholecalciferol ELISA Kit, Rat 1,25-dihydroxyvitamin D3 ELISA Kit, Rat DVD ELISA Kit, Rat DHVD 3 ELISA Kit

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Calcitriol concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcitriol, During the reaction, Calcitriol in the sample or standard competes with a fixed amount of biotin-labeled Calcitriol for sites on a pre-coated Monoclonal antibody specific to Calcitriol. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Calcitriol in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Materials and components

Reagent                                              Quantity

Assay plate (Item No. CELI-66118r-1)                                   1

Standard (Item No. CELI-66118r-2)                           2

Sample Diluent (Item No. CELI-66118r-3)                            1 × 20ml

Assay Diluent A (Item No. CELI-66118r-4)                                 1 × 10ml

Assay Diluent B (Item No. CELI-66118r-5)                                 1 × 10ml

Detection Reagent A (Item No. CELI-66118r-6)                         1 × 60μl

Detection Reagent B (Item No. CELI-66118r-7)                         1 × 120μl

Wash Buffer(25 x concentrate) (Item No. CELI-66118r-8)        1 × 30ml

Substrate (Item No. CELI-66118r-9)                              1 × 10ml

Stop Solution (Item No. CELI-66118r-10)                        1 × 10ml

Plate sealer                                             1

Manual                                                 1

 

Other supplies required

Microplate reader.

Pipettes and pipette tips.

EP tube

Deionized or distilled water.

 

Detection Range

15.6-1000 pg/mL.

 

TYPICAL DATA

This graph data is shown as an example. A standard curve must be generated each time the assay is run.
 

Concentration(pg/mL)

Mean OD450

1000                 

0.918

500

0.279

250

0.493

125

0.645

62.5

1.14

31.2

1.469

15.6

1.818

0

2.418

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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