Rat Cathepsin G ELISA Kit

Starting at: $756.00

  • Model: ELA-E1730r
  • 20 Units in Stock
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Rat Cathepsin G ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  CTSG

Protein Name  Cathepsin G

Alternative Name

Rat CTSG ELISA KIT ,Rat Cathepsin G ELISA KIT, Rat Vimentin-specific protease ELISA KIT, Rat vsp ELISA KIT

Intended use

Rat CATHEPSIN G ELISA KIT allows for the in vitro quantitative determination of CATHEPSIN G concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Rat CATHEPSIN G ELISA KIT has been pre-coated with an CATHEPSIN G antibody specific to CATHEPSIN G. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CATHEPSIN G and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CATHEPSIN G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CATHEPSIN G in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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