Rat CKMT1 (Creatine kinase U- type, mitochondrial) ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Rat Ckmt1 ELISA KIT ,Rat CKMT1A ELISA KIT ,Rat U-MtCK ELISA KIT ,Rat mia-CK ELISA KIT ,Rat acidic-type mitochondrial creatine kinase ELISA KIT ,Rat creatine kinase mitochondrial 1 (ubiquitous) ELISA KIT ,Rat ubiquitous mitochondrial creatine kinase ELISA KIT ,Rat creatine kinase mitochondrial 1A ELISA KIT

Intended use

Rat CKMT1 ELISA kit allows for the in vitro quantitative determination of Rat Creatine kinase U- type, mitochondrial concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Rat CKMT1 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CKMT1A, During the reaction, CKMT1A in the sample or standard competes with a fixed amount of biotin-labeled CKMT1A for sites on a pre-coated Monoclonal antibody specific to CKMT1A. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CKMT1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product    Min.Order 5kits

Delivery time 4-6month

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