Rat Corticotropin, ACTH ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Corticotropin,Adrenocorticotropic hormone, ACTH,AMSH,corticotropin-lipotropin,corticotropin-like intermediary peptide,CLIP,LPH,MSH,NPP,POC,adrenocorticotropic hormone,adrenocorticotropin,alpha-MSH,alpha-melanocyte-stimulating hormone,beta-LPH,beta-MSH,beta-endorphin,beta-melanocyte-stimulating hormone,gamma-LPH,gamma-MSH,lipotropin beta,lipotropin gamma,melanotropin alpha,melanotropin beta,melanotropin gamma,met-enkephalin,opiomelanocortin prepropeptide,pro-ACTH-endorphin,pro-opiomelanocortin,proopiomelanocortin preproprotein,proopiomelanocortin

Search name

Rat Corticotropin ELISA KIT ,Rat Adrenocorticotropic hormone ELISA KIT ,Rat  ACTH ELISA KIT ,Rat AMSH ELISA KIT ,Rat corticotropin-lipotropin ELISA KIT ,Rat corticotropin-like intermediary peptide ELISA KIT ,Rat CLIP ELISA KIT ,Rat LPH ELISA KIT ,Rat MSH ELISA KIT ,Rat NPP ELISA KIT ,Rat POC ELISA KIT ,Rat adrenocorticotropic hormone ELISA KIT ,Rat adrenocorticotropin ELISA KIT ,Rat alpha-MSH ELISA KIT ,Rat alpha-melanocyte-stimulating hormone ELISA KIT ,Rat beta-LPH ELISA KIT ,Rat beta-MSH ELISA KIT ,Rat beta-endorphin ELISA KIT ,Rat beta-melanocyte-stimulating hormone ELISA KIT ,Rat gamma-LPH ELISA KIT ,Rat gamma-MSH ELISA KIT ,Rat lipotropin beta ELISA KIT ,Rat lipotropin gamma ELISA KIT ,Rat melanotropin alpha ELISA KIT ,Rat melanotropin beta ELISA KIT ,Rat melanotropin gamma ELISA KIT ,Rat met-enkephalin ELISA KIT ,Rat opiomelanocortin prepropeptide ELISA KIT ,Rat pro-ACTH-endorphin ELISA KIT ,Rat pro-opiomelanocortin ELISA KIT ,Rat proopiomelanocortin preproprotein ELISA KIT ,Rat proopiomelanocortin ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Corticotropin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticotropin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Corticotropin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Corticotropin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Corticotropin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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