Rat Cytochrome c oxidase subunit 2, Mtco2 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Rat MTCO2 ELSIA KIT ,Rat mt-Co2 ELSIA KIT ,Rat COX-2 ELSIA KIT ,Rat COII ELSIA KIT ,Rat cytochrome c oxidase subunit II ELSIA KIT ,Rat Cytochrome c oxidase polypeptide II ELSIA KIT ,Rat Cytochrome c oxidase subunit 2 ELSIA KIT

Intended use

Rat Mtco2 ELISA kit allows for the in vitro quantitative determination of Rat Mtco2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Rat Mtco2 ELISA kit has been pre-coated with an antibody specific to Mtco2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mtco2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mtco2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mtco2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Rat Mtco2 ELISA kit Manual Request

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