Rat Cytochrome c oxidase subunit 3,MT-CO3 ELISA KIT

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  • Model: ELA-E10128r
  • 20 Units in Stock
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Rat Cytochrome c oxidase subunit 3, MT-CO3 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

MT-CO3 ,COIII,COXIII,MTCO3,COX3,MTCO3,COIII,cytochrome c oxidase subunit III,cytochrome c oxidase III,Cytochrome c oxidase subunit 3,Cytochrome c oxidase polypeptide III,

 

Search name

Rat MT-CO3 ELISA KIT ,Rat COIII ELISA KIT ,Rat COXIII ELISA KIT ,Rat MTCO3 ELISA KIT ,Rat COX3 ELISA KIT ,Rat MTCO3 ELISA KIT ,Rat COIII ELISA KIT ,Rat cytochrome c oxidase subunit III ELISA KIT ,Rat cytochrome c oxidase III ELISA KIT ,Rat Cytochrome c oxidase subunit 3 ELISA KIT ,Rat Cytochrome c oxidase polypeptide III ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Cytochrome c oxidase subunit 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome c oxidase subunit 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome c oxidase subunit 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome c oxidase subunit 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome c oxidase subunit 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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