Rat Decorin,DCN ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Rat DCN ELISA KIT ,Rat Decorin ELISA KIT ,Rat CSCD ELISA KIT ,Rat DSPG2 ELISA KIT ,Rat PG40 ELISA KIT ,Rat PGII ELISA KIT ,Rat PGS2 ELISA KIT ,Rat SLRR1B ELISA KIT ,Rat bone proteoglycan II ELISA KIT ,Rat dermatan sulphate proteoglycans II ELISA KIT ,Rat proteoglycan core protein ELISA KIT ,Rat small leucine-rich protein 1B ELISA KIT ,Rat Bone proteoglycan II ELISA KIT ,Rat PG-S2 ELISA KIT

Intended use

Rat DCN ELISA KIT allows for the in vitro quantitative determination of Rat Decorin concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Rat DCN ELISA KIT has been pre-coated with an antibody specific to Decorin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forDecorin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Decorin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Decorin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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