Rat Dentin sialophosphoprotein, DSPP ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Dentin sialophosphoprotein, Dentin sialoprotein,DSP, DSPP,DSP; DFNA39; DGI1; DMP3; DPP; DTDP2; dentin phosphophoryn; dentin phosphoprotein; dentin phosphoryn;

Search name

Rat Dentin sialophosphoprotein ELISA KIT ,Rat Dentin sialoprotein ELISA KIT ,Rat DSP ELISA KIT ,Rat  DSPP ELISA KIT ,Rat DSP ELISA KIT ,Rat DFNA39 ELISA KIT ,Rat DGI1 ELISA KIT ,Rat DMP3 ELISA KIT ,Rat DPP ELISA KIT ,Rat DTDP2 ELISA KIT ,Rat dentin phosphophoryn ELISA KIT ,Rat dentin phosphoprotein ELISA KIT ,Rat dentin phosphoryn ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Dentin sialophosphoprotein concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dentin sialophosphoprotein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Dentin sialophosphoprotein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dentin sialophosphoprotein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dentin sialophosphoprotein in the samples is then determined by comparing the O.D. of the samples to the standard curve.