Rat DNA topoisomerase 1, TOP1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

topoisomerase I,TOPI; DNA topoisomerase 1; type I DNA topoisomerase; topoisomerase (DNA) I,5.99.1.2,DNA topoisomerase 1),DNA topoisomerase I,

Search name

Rat TOP1 ELISA Kit, Rat DNA topoisomerase 1 ELISA Kit,Rattopoisomerase I ELISA KIT ,Rat TOPI ELISA KIT ,Rat DNA topoisomerase 1 ELISA KIT ,Rat type I DNA topoisomerase ELISA KIT ,Rat topoisomerase DNA I ELISA KIT ,Rat 5.99.1.2 ELISA KIT ,Rat DNA topoisomerase 1 ELISA KIT ,Rat DNA topoisomerase I ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat DNA topoisomerase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA topoisomerase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DNA topoisomerase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNA topoisomerase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DNA topoisomerase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.