Rat Ferritin Heavy Polypeptide,FTH ELISA Kit

Rat Ferritin Heavy Polypeptide, FTH ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Ferritin heavy chain,Ferritin H subunit,Cell proliferation-inducing gene 15 protein, FTH1,FHC; FTH; FTHL6; PIG15; PLIF; apoferritin; cell proliferation-inducing gene 15 protein; ferritin H subunit; ferritin heavy chain; placenta immunoregulatory factor; proliferation-inducing protein 15; ferritin, heavy polypeptide 1

Search name

Rat Ferritin heavy chain ELISA KIT ,Rat Ferritin H subunit ELISA KIT ,Rat Cell proliferation-inducing gene 15 protein ELISA KIT ,Rat  FTH1 ELISA KIT ,Rat FHC ELISA KIT ,Rat FTH ELISA KIT ,Rat FTHL6 ELISA KIT ,Rat PIG15 ELISA KIT ,Rat PLIF ELISA KIT ,Rat apoferritin ELISA KIT ,Rat cell proliferation-inducing gene 15 protein ELISA KIT ,Rat ferritin H subunit ELISA KIT ,Rat ferritin heavy chain ELISA KIT ,Rat placenta immunoregulatory factor ELISA KIT ,Rat proliferation-inducing protein 15 ELISA KIT ,Rat ferritin heavy polypeptide 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Ferritin Heavy Polypeptide,FTH concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FTH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FTH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FTH in the samples is then determined by comparing the O.D. of the samples to the standard curve.