Rat Gamma- aminobutyric acid ELISA Kit

Starting at: $756.00

  • Model: ELA-E0900r
  • 20 Units in Stock
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Rat Gamma- aminobutyric acid ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Gamma-Aminobutyric acid,γ-Aminobutyric acid, GABA

Search name
Rat GABA ELISA Kit, Rat Gamma- aminobutyric acid ELISA Kit, Rat γ-Aminobutyric acid ELISA Kit

Intended use
Rat Gamma- aminobutyric acid ELISA Kit allows for the in vitro quantitative determination of gamma-aminobutyric acid,Gamma-Aminobutyric acid
concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
Rat Gamma-aminobutyric acid ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Gamma-Aminobutyric acid, During the reaction, Gamma-Aminobutyric acid in the sample or
standard competes with a fixed amount of biotin-labeled Gamma-Aminobutyric acid for sites on a pre-coated Monoclonal antibody specific
to Gamma-Aminobutyric acid. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Gamma-Aminobutyric acid in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

Range 1.56-100ng/ml

Rat Gamma-aminobutyric acid ELISA Kit PDF manual download

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