Rat Glutamate decarboxylase 1, GAD1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Glutamate decarboxylase 1,67 kDa glutamic acid decarboxylase,GAD-67,Glutamate decarboxylase 67 kDa isoform, GAD1,glutamate decarboxylase 1 brain 67kDa; glutamate decarboxylase 1; CPSQ1; GAD; SCP; 67 kDa glutamic acid decarboxylase; GAD-67; glutamate decarboxylase 67 kDa isoform

Search name

Rat Glutamate decarboxylase 1 ELISA KIT ,Rat 67 kDa glutamic acid decarboxylase ELISA KIT ,Rat GAD-67 ELISA KIT ,Rat Glutamate decarboxylase 67 kDa isoform ELISA KIT ,Rat GAD1 ELISA KIT ,Rat glutamate decarboxylase 1 brain 67kDa ELISA KIT ,Rat glutamate decarboxylase 1 ELISA KIT ,Rat CPSQ1 ELISA KIT ,Rat GAD ELISA KIT ,Rat SCP ELISA KIT ,Rat 67 kDa glutamic acid decarboxylase ELISA KIT ,Rat GAD-67 ELISA KIT ,Rat glutamate decarboxylase 67 kDa isoform ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Glutamate decarboxylase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate decarboxylase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glutamate decarboxylase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glutamate decarboxylase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glutamate decarboxylase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.