Rat Glutamate receptor 1, Gria1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Gria1,GluR1,GLUH1; GLURA; GluA1; HBGR1; GluR-A ,AMPA 1; gluR-1; gluR-K1; glutamate receptor 1; glutamate receptor ionotropic AMPA 1,Glutamate receptor 1GluR-1,Glutamate receptor ionotropic, AMPA-selective glutamate receptor 1;
Search name
Rat Gria1 ELISA KIT ,Rat GluR1 ELISA KIT ,Rat GLUH1 ELISA KIT ,Rat GLURA ELISA KIT ,Rat GluA1 ELISA KIT ,Rat HBGR1 ELISA KIT ,Rat GluR-A ELISA KIT ,Rat AMPA 1 ELISA KIT ,Rat gluR-1 ELISA KIT ,Rat gluR-K1 ELISA KIT ,Rat glutamate receptor 1 ELISA KIT ,Rat glutamate receptor ionotropic AMPA 1 ELISA KIT ,Rat Glutamate receptor 1GluR-1 ELISA KIT ,Rat Glutamate receptor ionotropic ELISA KIT ,Rat AMPA-selective glutamate receptor 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat glutamate receptor 1,GluR-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GluR-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GluR-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GluR-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GluR-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
